Mol Biol Evol. The nine-spined stickleback is a nonmodel teleost, closely related Kawahara et al. The reference DNA samples were derived from peripheral blood of a individual, phenotypically normal male, and female controls with no detectable chromosomal aberrations by conventional karyotype analysis.
Am J Hum Genet ; 67 : — Therefore, microarray analysis in this patient not only confirmed the cytogenetic abnormality with more precision but also identified a cryptic deletion that was not identified by routine high-resolution chromosome analysis.
Genome Res. In all further comparisons, only these 10, sequences were utilized. Bell et al. In the sex-average linkage map of the HP cross, 5, reference sequences with 5, SNPs mapped to the three-spined stickleback genome table 3 indicating a high degree of genomic synteny between nine- and three-spined sticklebacks fig.
Genome Research. Any clone that hybridized to multiple chromosomal locations, likely reflecting paralogous sequences, was excluded from the array. This effect is independent of the distribution of double-strand breaks DSBs in male meiosis reported by Brick et al. The results are presented in File S4.
Journal of Medical Genetics Science 34 : However, 16 cold regions are completely devoid of segmental duplication.
To determine whether the cold regions seen in the G 2 :F 1 population are cold in other populations, we examined these regions in the HS used to construct a recent linkage map of the mouse Cox et al. Nucleic Acids Res ; 30 : e The distribution of crossovers in males was compared to the distribution of recombination hotspots identified through high-resolution analysis of DSBs in Smagulova et al.
Twenty-four lanes were used for the HR cross and 30 for the HP cross sequencing. The sex difference is also observed in the distribution of total number of crossovers per individual Figure S3.